Techniques of fragile renal organoids transplantation in mice

ABSTRACT Purpose: This study aimed to develop a microsurgical technique to transplant extremely fragile renal organoids in vivo, created by in-vitro reaggregation of metanephros from fetal mice.These organoids in reaggregation and development were examined histologically after transplantation under the renal capsule.Methods: Initially, metanephros

read more


Transcript, protein, metabolite and cellular studies in skin fibroblasts demonstrate variable pathogenic impacts of NPC1 mutations

Abstract Background Niemann-Pick type C (NP-C) is a rare neurovisceral genetic disorder caused by mutations in the NPC1 or the NPC2 gene.NPC1 is a multipass-transmembrane protein essential for egress of cholesterol from late endosomes/lysosomes.To evaluate impacts of NPC1 mutations, we examined fibroblast cultures from 26 NP-C1 patients with clinic

read more

Acute stress response of fathead minnows caged downstream of municipal wastewater treatment plants in the Bow River, Calgary.

We examined whether exposure to municipal wastewater effluent (MWWE) compromised the stress performance of laboratory-reared fathead minnows (Pimephales promelas) in a field setting.Adult minnows were caged at two sites upstream and three sites downstream of wastewater treatments plants (WWTPs) discharging MWWE into the Bow River, Calgary, Alberta,

read more

Auditory Streaming Complexity and Renaissance Mass Cycles

How did Renaissance listeners experience the polyphonic mass ordinary cycle in the soundscape of the church? We hypothesize that the textural differences in complexity between mass movements allowed listeners to track the progress of the service, regardless of intelligibility of the text or sophisticated musical knowledge.Building on the principles

read more